High Prevalence ofEscherichia coli-Producing CTX-M-15 Extended-Spectrum Beta-Lactamases in Poultry and Human Clinical Isolates in Romania

• DOI: 10.1089/mdr.2014.0248
• Publication Date: 2015-03-03T16:34:42Z
• Authors: Iuliana E. Maciuca; Nicola J. Williams; Cristina Tuchilus; Olivia Dorneanu; Eleonora Guguianu; Catalin Carp-Carare; Cristina Rimbu; Dorina Timofte
• Keywords: Multilocus sequence typing; Beta-lactamase

Use of antibiotics in food animals may contribute to development and spread resistant organisms, particularly so some countries. The aim this study was two-fold; first, establish the prevalence extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli chicken production a region within Romania. Second, relatedness ESBL-producing E. isolates recovered from broilers, abattoir workers where chickens were slaughtered human clinical specimens two regional hospitals. results indicated very high (69%) rate carriage ESBL AmpC-producing with 36% CTX-M producers. Sequencing showed that Romania have highest worldwide (53%) blaCTX-M-15 reported poultry isolates. majority cephalosporin-resistant carried plasmid-mediated blaampC genes, mostly blaCMY-2 type, one prevalences Europe. predominant type found most coharbored blaOXA-1, blaTEM, aac(6′)-ib-cr. (60%) belonged pandemic virulent clone B2-ST131. clonal relationship between broiler CTX-M-producing assessed by macrorestriction pulsed-field gel electrophoresis (PFGE) multilocus sequence typing (MLST), which strain diversity no common STs Moreover, IncI1 prevalent replicon also transconjugants, indicating plasmids not play role transfer blaCTX-M genes. This identifies occurrence incidence blaCTX-M-15, reflects main infections country.