Genomic Analysis of Multidrug-Resistant Enterococcus faecium Harboring vanA Gene from Wastewater Treatment Plants

• DOI: 10.1089/mdr.2021.0254
• Publication Date: 2022-02-16T23:07:20Z
• Authors: Beatriz Oliveira ...; Kayo Bianco; Ana Paula Alves N...; Andressa Silva Go...; Thais Costa Moreira; Maysa Mandetta Cl...
• Keywords: Enterococcus faecium; Multilocus sequence typing; Genomic island

The emergence of vancomycin-resistant Enterococcus faecium (Efm) harboring vanA gene and multidrug-resistant determinants is a relevant public health concern. It an opportunistic pathogen responsible for nosocomial infections widely distributed in the environment, including wastewater treatment plants (WWTPs). Our study addresses genomic investigation vanA-carrying Efm from WWTPs Brazil. Samples five supplied with sewage different sources were evaluated. Here we present whole-genome sequencing eight vanA-Efm isolates performed on Illumina MiSeq platform. All these presented profile, strains treated wastewater. Multiple antimicrobial resistance genes (ARGs) found, such as aph(3')-IIIa, ant(6')-Ia, erm(B), msrC, some them being allocated plasmids. virulence profile was predominantly constituted by efaAfm acm all isolates, except one, predicted human pathogens. Multilocus sequence typing analysis revealed new allele STs, three previously described (ST32, ST168, ST253) two novel ones (ST1893 ST1894). Six belonged to CC17, often associated hospital outbreaks. As far our knowledge, no studies recovered belonging CC17 Therefore, findings point environmental spread carrying multiple ARGs.