Adjuvant Properties of Cytosine-Phosphate-Guanosine Oligodeoxynucleotide in Combination with Various Polycations in an Ovalbumin-Vaccine Model

• DOI: 10.1089/nat.2011.0291
• Publication Date: 2011-07-28T09:11:08Z
• Authors: Sylvie Maubant; Claire Banissi; Samantha Beck; Anne Chauvat; Antoine F. Carpen...
• Keywords: CpG Oligodeoxynucleotide; CpG site

Oligonucleotides containing CpG motifs (cytosine-phosphate-guanosine oligodeoxynucleotide [CpG ODN]) display strong immunostimulatory effects, and polycations have been previously reported as cellular delivery system. In the present study, we investigated adjuvant properties of combinations a ODN with various (poly-arginine, poly-lysine, poly-histidine, or chitosan) in an ovalbumin vaccination model. We showed that, when combined to ODN, poly-arginine but not poly-lysine chitosan, enhanced efficiently both IgG antibody production number splenocytes secreting interferon-gamma after stimulation CD8+ T cell-restricted peptide. Interestingly, ODN-poly-arginine, which was most efficient, compared favorably complete Freund's aluminium salts induced no local toxicity, making this combination very attractive for vaccines.