ERK1/2-Mediated Schwann Cell Proliferation in the Regenerating Sciatic Nerve by Treadmill Training

• DOI: 10.1089/neu.2008.0711
• Publication Date: 2009-10-15T18:33:31Z
• Authors: Tae Beom Seo; Myung-Jin Oh; Byoung-Gun You; Ku-Birm Kwon; In-Ae Chang; Jin-Hwan Yoon; Chan-Yong Lee; Uk Namgung
• Keywords: Schwann cell; Dorsal root ganglion; Neurite; Peripheral nerve injury; Sciatic nerve injury; Nerve Injury; Gap-43 protein

Proliferation of Schwann cells in the injured peripheral nerve supports axonal regeneration, and physical training experimental animals has been shown to promote regeneration. Extracellular signal-regulated kinase 1/2 (ERK1/2) activity can mediate neuronal responses lesion signals, but its role non-neuronal area is largely unknown. Here we report that treadmill (TMT) facilitates regeneration via upregulation phospho-ERK1/2 protein levels sciatic nerve. Low-intensity, not high-intensity, TMT increased neurite outgrowth dorsal root ganglion (DRG) sensory neurons potentiated cell proliferation. elevated GAP-43 mRNA protein, nerves. also enhanced phospho-c-Jun In-vivo administration ERK1/2 inhibitor PD98059 eliminated phospho-c-Jun, suggesting phosphorylation c-Jun protein. treatment decreased BrdU-labeled proliferating distal portion nerve, delayed regrowth was promoted by TMT. The present data suggest may play an important TMT-mediated enhancement