An Endosome-to-Plasma Membrane Pathway Involved in Trafficking of a Mutant Plasma Membrane ATPase in Yeast

• DOI: 10.1091/mbc.11.2.579
• Publication Date: 2013-07-02T23:47:08Z
• Authors: Wen-jie Luo; Amy Chang
• Keywords: Cell membrane

The plasma membrane ATPase, encoded by PMA1, is delivered to the cell surface via secretory pathway. Previously, we characterized a temperature-sensitive pma1 mutant in which newly synthesized Pma1-7 not but mislocalized instead vacuole at 37°C. Severalvps mutants, are defective vacuolar protein sorting, suppress targeting-defective allowing Pma1 move once again membrane. In this study, have analyzed trafficking endosomal system monitoring movement of vps36, vps1, andvps8 mutants. Upon induction expression, accumulates prevacuolar compartment vps36cells. After chase, fraction both vps1 cells, appears small punctate structures before arrival surface. Nevertheless, biosynthetic traffic follow different routes vps8 and vps1: protein-sorting receptor Vps10p stable vps1. Furthermore, defect endocytic delivery was revealed (andvps36) endocytosis bulk marker FM 4-64. Moreover, there down-regulation from mating Ste3, consistent with persistent recycling an These data support model diverted Golgi invps1 cells. We hypothesize that vps8and contrast moves intermediates, implicating endosome-to-surface