Functional Significance of the Specific Sites Phosphorylated in Desmin at Cleavage Furrow: Aurora-B May Phosphorylate and Regulate Type III Intermediate Filaments during Cytokinesis Coordinatedly with Rho-kinase

• DOI: 10.1091/mbc.e02-09-0612
• Publication Date: 2003-04-09T18:54:47Z
• Authors: Aie Kawajiri; Yoshihiro Yasui; Hidemasa Goto; Masaaki Tatsuka; Masahide Takahashi; Koh-ichi Nagata; Masaki Inagaki
• Keywords: Cleavage furrow; Aurora B kinase; Desmin; Aurora inhibitor; Cleavage (geology)

Aurora-B is a protein kinase required for chromosome segregation and the progression of cytokinesis during cell cycle. We report here that phosphorylates GFAP desmin in vitro, this phosphorylation leads to reduction filament forming ability. The sites phosphorylated by Aurora-B; Thr-7/Ser-13/Ser-38 GFAP, Thr-16 are common with those related Rho-associated (Rho-kinase), which has been reported phosphorylate at cleavage furrow cytokinesis. identified Ser-59 be specific site vitro. Use an antibody specifically recognized led finding also Saos-2 cells. Desmin mutants, vitro and/or Rho-kinase changed Ala or Gly, cause dramatic defects separation between daughter cells results presented suggest possibility may regulate furrow-specific type III IFs coordinatedly