Diverse and Specific Gene Expression Responses to Stresses in Cultured Human Cells

• DOI: 10.1091/mbc.e03-11-0799
• Publication Date: 2004-03-09T01:53:34Z
• Authors: John Isaac Murray; Michael L. Whitfield; Nathan D. Trinklein; Richard M. Myers; Patrick O. Brown; David Botstein
• Keywords: HeLa

We used cDNA microarrays in a systematic study of the gene expression responses HeLa cells and primary human lung fibroblasts to heat shock, endoplasmic reticulum stress, oxidative crowding. Hierarchical clustering data revealed groups genes with coherent biological themes, including that responded specific stresses others multiple types stress. Fewer increased after than free-living yeasts, which have large general stress response program. Most induced by diverse are involved cell-cell communication other processes higher organisms. found substantial differences between fibroblasts. For example, many were dithiothreitol but not cells; conversely, group transcription factors, c-fos c-jun, shock The dataset is freely available for search download at http://microarray-pubs.stanford.edu/human_stress/Home.shtml.