The Endo-Lysosomal Sorting Machinery Interacts with the Intermediate Filament Cytoskeleton

• DOI: 10.1091/mbc.e04-03-0272
• Publication Date: 2004-09-30T00:24:21Z
• Authors: Melanie L. Styers; Gloria Salazar; Rachal Love; Andrew A. Peden; Andrew P. Kowalczyk; Victor Faundez
• Keywords: Organelle; Transport protein; Intermediate Filament Protein

Cytoskeletal networks control organelle subcellular distribution and function. Herein, we describe a previously unsuspected association between intermediate filament proteins the adaptor complex AP-3. AP-3 cosedimented coimmunoprecipitated as free of microtubule actin binding proteins. Genetic perturbation cytoskeleton triggered changes in late endocytic/lysosome compartments. Concomitant with these architectural changes, similarly to AP-3-null mocha cells, fibroblasts lacking vimentin were compromised their vesicular zinc uptake, organellar pH, total surface content cargoes. However, levels, well transferrin receptor, membrane protein whose sorting is independent, remained unaltered both AP-3- vimentin-null cells. Based on phenotypic convergence deficiencies, predicted documented reduced autophagosome phenotype reported cells disrupted cytoskeletons. Our results reveal novel role organelle/adaptor positioning regulation