Regulation of the Formin for3p by cdc42p and bud6p

• DOI: 10.1091/mbc.e07-02-0094
• Publication Date: 2007-08-16T00:18:40Z
• Authors: Sophie G. Martin; Sergio A. Rincón; Roshni Basu; Pilar Pérez; Fred Chang
• Keywords: Formins; CDC42; Cell polarity

Formins are conserved actin nucleators responsible for the assembly of diverse structures. Many formins controlled through an autoinhibitory mechanism involving interaction a C-terminal DAD sequence with N-terminal DID sequence. Here, we show that fission yeast formin for3p, which mediates cable and polarized cell growth, is regulated by similar in vivo. Multiple sites govern for3p localization to tips. The activity inhibited intramolecular divergent DID-like sequences. A mutant expressed at endogenous levels produces more robust cables, appear have normal organization dynamics. We identify cdc42p as primary Rho GTPase involved regulation. Both cdc42p, binds N terminus bud6p, near DAD-like sequence, needed full activity, but mutation restores other phenotypes cdc42 bud6 mutants. In particular, suppresses bipolar growth (NETO) defect bud6Delta cells. These findings suggest bud6p activate relieving autoinhibition.