Cleavage of Stalled Forks by Fission Yeast Mus81/Eme1 in Absence of DNA Replication Checkpoint

• DOI: 10.1091/mbc.e07-07-0728
• Publication Date: 2007-11-22T02:05:52Z
• Authors: Benoît Froget; Joël Blaisonneau; Sarah Lambert; Giuseppe Baldacci
• Keywords: Schizosaccharomyces; Replication

During replication arrest, the DNA checkpoint plays a crucial role in stabilization of replisome at stalled forks, thus preventing collapse active forks and formation aberrant structures. How this acts to preserve integrity structures fork is poorly understood. In Schizosaccharomyces pombe, kinase Cds1 negatively regulates structure-specific endonuclease Mus81/Eme1 genomic when perturbed. Here, we report that, response hydroxyurea (HU) treatment, prevents S-phase-specific breakage resulting from Mus81 nuclease activity. However, loss regulation by not sufficient produce HU-induced breaks. Our results suggest that unscheduled cleavage permitted stabilized checkpoint. We also show breaks are partially dependent on Rqh1 helicase, fission yeast homologue BLM, but independent its helicase This suggests efficient requires Rqh1. Finally, identified an interplay between activity Chk1-dependent damage during S-phase have collapsed.