Arp2 Links Autophagic Machinery with the Actin Cytoskeleton

• DOI: 10.1091/mbc.e07-09-0892
• Publication Date: 2008-02-21T02:23:30Z
• Authors: Iryna Monastyrska; Congcong He; Jiefei Geng; Adam D. Hoppe; Zhijian Li; Daniel J. Klionsky
• Keywords: Organelle; Transport protein

Macroautophagy involves lysosomal/vacuolar elimination of long-lived proteins and entire organelles from the cytosol. The process begins with formation a double-membrane vesicle that sequesters bulk cytoplasm, or specific cargo destined for delivery. completed fuses lysosome/vacuole limiting membrane, releasing its content into organelle lumen subsequent degradation recycling resulting macromolecules. A majority autophagy-related (Atg) are required at step formation. integral membrane protein Atg9 cycles between certain intracellular compartments nucleation site, presumably to supply membranes necessary macroautophagic In this study we have tracked movement over time in living cells by using real-time fluorescence microscopy. Our results reveal an actin-related protein, Arp2, briefly colocalizes directly regulates dynamics movement. We propose Arp2/3 complex regulate transport types autophagy.