Aurora-C Kinase Deficiency Causes Cytokinesis Failure in Meiosis I and Production of Large Polyploid Oocytes in Mice

• DOI: 10.1091/mbc.e10-02-0170
• Publication Date: 2010-05-19T22:37:01Z
• Authors: Kuo-Tai Yang; Shu-Kuei Li; Chih-Chieh Chang; Chieh-Ju C. Tang; Yi-Nan Lin; Sheng-Chung Lee; Tang K. Tang
• Keywords: Midbody; Meiosis II; Aurora B kinase; Prometaphase

We previously isolated Aurora-C/Aie1 in a screen for kinases expressed mouse sperm and eggs. Here, we show the localization of endogenous Aurora-C examine its roles during female meiosis. was detected at centromeres along chromosome arms prometaphase I-metaphase I concentrated metaphase II, which also phosphorylated Thr171. During anaphase I-telophase transition, dephosphorylated relocalized to midzone midbody. Microinjection kinase-deficient (AurC-KD) mRNA into oocytes significantly inhibited activity caused multiple defects, including misalignment, abnormal kinetochore-microtubule attachment, premature segregation, cytokinesis failure meiosis I. Furthermore, AurC-KD reduced histone H3 phosphorylation kinetochore Bub1 BubR1. Similar effects were observed injected with INCNEP-delIN mRNAs, binding motif removed. The most dramatic effect AurC-KD-injected is I, resulting producing large polyploid oocytes, pattern similar deficiency human spermatozoa. Surprisingly, no Aurora-B protein oocytes. propose that Aurora-C, but not Aurora-B, plays essential