Specific deletion ofCdc42does not affect meiotic spindle organization/migration and homologous chromosome segregation but disrupts polarity establishment and cytokinesis in mouse oocytes

• DOI: 10.1091/mbc.e13-03-0123
• Publication Date: 2013-10-17T04:37:37Z
• Authors: Zhen-Bo Wang; Zong-Zhe Jiang; Qing-Hua Zhang; Meng-Wen Hu; Lin Huang; Xiang-Hong Ou; Lei Guo; Ying-Chun Ouyang; Yi Hou; Cord Brakebusch; Heide Schatten; Qing-Yuan Sun
• Keywords: Polar body; Oocyte activation; Cell polarity; CDC42; Meiosis II

Mammalian oocyte maturation is distinguished by highly asymmetric meiotic divisions during which a haploid female gamete produced and almost all the cytoplasm maintained in egg for embryo development. Actin-dependent meiosis I spindle positioning to cortex induces formation of polarized actin cap polarity, it determines resulting two polar bodies. Here we investigate functions Cdc42 oocyte-specific deletion through Cre-loxP conditional knockout technology. We find that causes infertility mice. has little effect on organization migration but inhibits body emission, although homologous chromosome segregation occurs. The failure cytokinesis due loss Arp2/3 accumulation formation; thus defective contract ring. In addition, correlate active dynamics with its function emission relationship between as well mouse oocytes.