Structure and function of Spc42 coiled-coils in yeast centrosome assembly and duplication

• DOI: 10.1091/mbc.e19-03-0167
• Publication Date: 2019-04-10T18:33:38Z
• Authors: Amanda C. Drennan; Shivaani Krishna; Mark A. Seeger; Michael P. Andreas; Jennifer M. Gardner; Emily K. R. Sether; Sue L. Jaspersen; Ivan Rayment
• Keywords: Coiled coil; Spindle pole body; Antiparallel (mathematics); Organelle

Centrosomes and spindle pole bodies (SPBs) are membraneless organelles whose duplication assembly is necessary for bipolar mitotic formation. The structural organization functional roles of major proteins in these can provide critical insights into cell division control. Spc42, a phosphoregulated protein with an N-terminal dimeric coiled-coil (DCC), assembles hexameric array at the budding yeast SPB core, where it functions as scaffold assembly. Here, we present vitro vivo data to elucidate arrangement biological Spc42 elements. Crystal structures reveal details two additional coiled-coils Spc42: central trimeric C-terminal antiparallel DCC. Contributions three adjacent undetermined regions formation ∼145 Å lattice lipid monolayer assay redundancy We propose updated model that incorporates inherent symmetry elements lattice, thereby establishes observed sixfold symmetry. implications this core layer discussed.