PP2A-B56 regulates Mid1 protein levels for proper cytokinesis in fission yeast

• DOI: 10.1091/mbc.e24-08-0382
• Publication Date: 2025-03-05T17:25:11Z
• Authors: Madeline L. Chrup...; James B. Moseley
• Keywords: Septin; Schizosaccharomyces

Protein phosphorylation regulates many steps in the cell division process including cytokinesis. In fission yeast cells, anillin-like protein Mid1 sets plane and is regulated by phosphorylation. Multiple kinases act on Mid1, but no phosphatases have been shown to regulate Mid1. Here, we discovered that conserved phosphatase PP2A-B56 required for proper cytokinesis promoting levels. We find par1∆ cells lacking primary B56 subunit divide asymmetrically due assembly of misplaced cytokinetic rings slide towards tips. These mutants reduced whole-cell levels protein, leading at ring. Restoring expression suppresses defects. This work identifies a new pathway regulating through with implications control other organisms.