Genome-wide identification of target genes repressed by the zinc finger transcription factor REST/NRSF in the HEK 293 cell line

• DOI: 10.1093/abbs/gmp095
• Publication Date: 2009-11-12T01:28:04Z
• Authors: Zhihui Liu; Ming Liu; Gang Niu; Yi Cheng; Jian Fei
• Keywords: Chromatin immunoprecipitation; HEK 293 cells

Transcriptional repression is as important transcriptional activation in establishing cell-type specific patterns of gene expression. RE1-silencing transcription factor (REST), also known neuronal restrictive silencing (NRSF), a regulator that represses battery differentiation genes non-neuronal cells or neural progenitor by binding to DNA sequence (repressor element-1/neuron-restrictive silencer element, RE1/NRSE). REST/NRSF functions the development are widely studied, however, little about target various lineages may result cell differentiation. Here, we use RNA interference (RNAi) technology combined with microarray strategy identify potential targets and RE1/NRSEs human line HEK 293. Expression 54 was up-regulated inhibition 293 according experiment 13 those were further confirmed quantitative RT-PCR. Our results good confidence reliability current research data based on silico, chromatin immunoprecipitation combination microarrays (ChIP-chip), high-throughput sequencing (ChIP-seq). However, view fact thousands have been testified predicted be recognized REST/NRSF, our show only few among directly interaction sites sequences.