EZH2 induces the expression of miR-1301 as a negative feedback control mechanism in triple negative breast cancer

• DOI: 10.1093/abbs/gmy050
• Publication Date: 2018-04-17T19:09:12Z
• Authors: Qiuju Wu; Zekun Chen; Guihua Zhang; Wenhui Zhou; You Peng; Rong Liu; Ceshi Chen; Jing Feng
• Keywords: Triple-negative breast cancer

Breast cancer is one of the most common malignancies in women. ERα, PR, and HER2 triple negative breast (TNBC) current research focus because lack effective targeted therapies. In our study, lentivirus systems were used to overexpress EZH2 miR-1301 TNBC cell lines. Western blot analysis RT-qPCR detect protein microRNA levels. The TCGA Kaplan Meier plotter databases analyze expression levels cancer. effect overexpression on proliferation, migration colony formation determined by using sulforhodamine B (SRB) assay, wound healing assay respectively. Furthermore, an xenograft mouse model was investigate function vivo. Finally, dual luciferase reporter verify binding site miR-1301. We found that induced two lines, HCC1937 HCC1806. Overexpression suppressed formation, as well tumor growth immunodeficient mice. Interestingly, inhibited 3'-UTR gene. These data suggest induces a feedback control mechanism TNBC.