In situ analysis of foliar zinc absorption and short-distance movement in fresh and hydrated leaves of tomato and citrus using synchrotron-based X-ray fluorescence microscopy

• DOI: 10.1093/aob/mcu212
• Publication Date: 2014-11-16T01:10:45Z
• Authors: Yumei Du; Peter M. Kopittke; Barry N. Noller; Simon A. James; Hugh H. Harris; Zhi Ping Xu; Peng Li; David R. Mulligan; Longbin Huang
• Keywords: Limiting; X-Ray Fluorescence

Background and Aims Globally, zinc deficiency is one of the most important nutritional factors limiting crop yield quality. Despite widespread use foliar-applied fertilizers, much remains unknown regarding movement from foliar surface into vascular structure for translocation other tissues key affecting this diffusion. Methods Using synchrotron-based X-ray fluorescence microscopy (µ-XRF), absorption nitrate or hydroxide was examined in fresh leaves tomato (Solanum lycopersicum) citrus (Citrus reticulatus). Key Results The increased concentrations underlying by up to 600-fold but only 5-fold citrus. magnitude influenced form applied, status treated leaf which it applied (abaxial adaxial). Once had moved through appeared bind strongly, with limited further redistribution. Regardless this, these lower-order veins, 2- 10-fold higher than adjacent tissues. However, even once higher-order still comparatively limited, decreasing levels similar background within 1–10 mm. Conclusions results advance our understanding that influence efficacy fertilizers demonstrate merits an innovative methodology studying mechanisms.