Zepyros: a webserver to evaluate the shape complementarity of protein–protein interfaces

• DOI: 10.1093/bioadv/vbaf051
• Publication Date: 2025-03-22T21:16:15Z
• Authors: Mattia Miotto; Lorenzo Di Rienzo; Leonardo Bo’; Giancarlo Ruocco; Edoardo Milanetti

Abstract

Motivation Shape complementarity of molecular surfaces at the interfaces is a well-known characteristic of protein–protein binding regions, and it is critical in influencing the stability of the complex. Measuring such complementarity is of great importance for a number of theoretical and practical implications; however, only a limited number of tools are currently available to efficiently and rapidly assess it.

Results Here, we introduce Zepyros (ZErnike Polynomials analYsis of pROtein Shapes), a webserver for fast measurement of the shape complementarity between two molecular interfaces of a given protein–protein complex using structural information. Zepyros is implemented as a publicly available tool with a user-friendly interface.

Availability and implementation Our server can be found at the following link (all major browser supported): https://zepyros.bio-groups.com.