Ustiloxins, fungal cyclic peptides, are ribosomally synthesized in Ustilaginoidea virens

• DOI: 10.1093/bioinformatics/btu753
• Publication Date: 2014-11-21T01:18:58Z
• Authors: Takahiro Tsukui; Nozomi Nagano; Myco Umemura; Toshitaka Kumagai; Goro Terai; Masayuki Machida; Kiyoshi Asai
• Keywords: Gene cluster

Abstract Motivation: Ustiloxins A and B are toxic cyclic tetrapeptides, Tyr-Val/Ala-Ile-Gly (Y-V/A-I-G), that were originally identified from Ustilaginoidea virens, a pathogenic fungus affecting rice plants. Contrary to our report ustiloxin is ribosomally synthesized in Aspergillus flavus, recent suggested ustiloxins by non-ribosomal peptide synthetase U.virens. Thus, we analyzed the U.virens genome, identify responsible gene cluster. Results: The biosynthetic cluster was genome of based on homologies ribosomal for A.flavus. It contains encoding precursor protein having five Tyr-Val-Ile-Gly three Tyr-Ala-Ile-Gly motifs B, respectively, strongly indicating synthesized. Availability implementation: Accession codes A.flavus clusters NCBI BR001221 BR001206, respectively. Contact: [e-mail] or [e-mail] Supplementary information: data available at Bioinformatics online.