LongISLND: in silico sequencing of lengthy and noisy datatypes

• DOI: 10.1093/bioinformatics/btw602
• Publication Date: 2016-09-26T00:07:40Z
• Authors: Bayo Lau; Marghoob Mohiyuddin; John C. Mu; Li Tai Fang; Narges Bani Asadi; Carolina Dallett; Hugo Y. K. Lam
• Keywords: 0206 medical engineering; Computational Biology; High-Throughput Nucleotide Sequencing; Computer Simulation; 02 engineering and technology; Applications Notes; Sequence Alignment; Software

LongISLND is a software package designed to simulate sequencing data according the characteristics of third generation, single-molecule technologies. The general architecture easily extendable, as demonstrated by emulation Pacific Biosciences (PacBio) multi-pass with P5 and P6 chemistries, producing in FASTQ, H5, latest PacBio BAM format. We demonstrate its utility downstream processing consensus building variant calling.LongISLND implemented Java available at http://bioinform.github.io/longislnd CONTACT: [e-mail] information: Supplementary are Bioinformatics online.