Real-time demultiplexing Nanopore barcoded sequencing data with npBarcode

• DOI: 10.1093/bioinformatics/btx537
• Publication Date: 2017-08-23T11:15:39Z
• Authors: Son Hoang Nguyen; Tania P S Duarte; Lachlan J M Coin; Minh Duc Cao
• Keywords: Nanopore

The recent introduction of a barcoding protocol for Oxford Nanopore sequencing has increased the versatility technology. Several bioinformatics tools have been developed to demultiplex barcoded reads, but none them supports streaming analysis. This limits use multiplexed in real-time applications, which is one main advantages technology.We introduced npBarcode, an open source and cross-platform tool barcode demultiplexing fashion that can be used pipe data further analyses. also provides friendly graphical user interface by integrating module into npReader, making possible monitor progress concurrently when still progress. We show our algorithm achieves accuracies at least as good competing tools.npBarcode bundled Japsa-a Java kit genome analysis, freely available https://github.com/mdcao/[e-mail] or [e-mail] are Bioinformatics online.