CRISPR-Local: a local single-guide RNA (sgRNA) design tool for non-reference plant genomes

• DOI: 10.1093/bioinformatics/bty970
• Publication Date: 2018-11-29T13:34:53Z
• Authors: Jiamin Sun; Hao Liu; Jianxiao Liu; Shikun Cheng; Yong Peng; Qinghua Zhang; Jianbing Yan; Hai-Jun Liu; Ling-Ling Chen
• Keywords: Guide RNA; Subgenomic mRNA

CRISPR-Local is a high-throughput local tool for designing single-guide RNAs (sgRNAs) in plants and other organisms that factors genetic variation optimized to generate genome-wide sgRNAs. outperforms sgRNA design tools the following respects: (i) sgRNAs suitable non-reference varieties; (ii) screening are capable of simultaneously targeting multiple genes; (iii) saving computational resources by avoiding repeated calculations from submissions (iv) running offline, with both command-line graphical user interface modes ability export formats further batch analysis or visualization. We have applied 71 public plant genomes, using CRISPR/Cas9 CRISPR/cpf1 systems.