SpaCell: integrating tissue morphology and spatial gene expression to predict disease cells

• DOI: 10.1093/bioinformatics/btz914
• Publication Date: 2019-12-04T12:11:06Z
• Authors: Xiao Tan; Andrew Su; Minh Tran; Quan Nguyen
• Keywords: Statistics and Probability; 0301 basic medicine; 0303 health sciences; 1303 Biochemistry; Computational Biology; Gene Expression; Biochemistry; Computer Science Applications; 004; Computational Mathematics; 03 medical and health sciences; Computational Theory and Mathematics; 1312 Molecular Biology; 1706 Computer Science Applications; 2613 Statistics and Probability; Molecular Biology; 2605 Computational Mathematics; Software; 1703 Computational Theory and Mathematics

Abstract

Motivation Spatial transcriptomics (ST) technology is increasingly being applied because it enables the measurement of spatial gene expression in an intact tissue along with imaging morphology of the same tissue. However, current analysis methods for ST data do not use image pixel information, thus missing the quantitative links between gene expression and tissue morphology.

Results We developed a user-friendly deep learning software, SpaCell, to integrate millions of pixel intensity values with thousands of gene expression measurements from spatially barcoded spots in a tissue. We show the integration approach outperforms the use of gene-count data alone or imaging data alone to build deep learning models to identify cell types or predict labels of tissue images with high resolution and accuracy.

Availability and implementation The SpaCell package is open source under an MIT licence and it is available at https://github.com/BiomedicalMachineLearning/SpaCell.

Supplementary information Supplementary data are available at Bioinformatics online.