Principal component analysis, a useful tool to study cyclin-dependent kinase-inhibitor’s effect on cerebral ischaemia

• DOI: 10.1093/braincomms/fcaa136
• Publication Date: 2020-08-31T18:24:51Z
• Authors: Lucas Le Roy; Ahmed Amara; Cloé Le Roux; Ozvan Bocher; Anne Létondor; Nathalie Benz; Serge Timsit
• Keywords: Evans Blue; Extravasation

Abstract Stroke is a leading cause of acute death related in part to brain oedema, blood–brain barrier disruption and glial inflammation. A cyclin-dependant kinase inhibitor, (S)-roscovitine, was administered 90 min after onset on model rat focal cerebral ischaemia. Brain swelling Evans Blue tissue extravasation were quantified injection. Combined fluorescence immunofluorescence endothelial cells (RECA1), microglia (isolectin-IB4) astrocytes (glial fibrillary acidic protein) analysed. Using Student’s t-test or Mann–Whitney test, (S)-roscovitine improved recovery by more than 50% compared vehicle (Mann–Whitney, P < 0.001), decreased significantly (t-test, = 0.0128) mostly the rostral brain. Main analysis therefore performed cut for maximize biological observations (cut B). group (60%, t-test, 0.049) further supported spectrophotometer 0.0002) macroscopic photonic 0.07). An increase RECA-1 intensity observed ischaemic hemisphere non-ischaemic hemisphere. Further study showed, that treated vehicle, showed decrease of: (i) about 20% globally, mainly located cortex (−28.5%, 0.03); (ii) Microglia’s number 55% 0.006) modulated reactive through trend toward less (15%, 0.05) astrogliosis (21%, 0.076). To decipher complex relationship these components, we analysed six quantitative variables our principal component from studies same animals. Principal differentiated non-treated animals dimension 1 with negative values animals, positive Interestingly, stroke presented correlation this dimension, while all other correlation. Dimensions 2 allowed identification two groups co-varying variables: cells, both dimensions, astrocyte number, 2. This partition suggests different mechanisms. Correlation matrix concordant results. Because its pleiotropic action elements NeuroVascular Unit response, may represent an effective treatment against oedema stroke.