Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing

• DOI: 10.1093/burnst/tkab033
• Publication Date: 2021-11-09T20:17:02Z
• Authors: Xiaoxuan Lei; Liuhanghang Cheng; Yu Yang; Mengru Pang; Yunqing Dong; Xuanru Zhu; Caihong Chen; Zexin Yao; Gang Wu; Biao Cheng; Tymour Forouzanfar
• Keywords: Submucosa

Abstract Background Acute skin wounds may compromise the barrier, posing a risk of infection. Small intestinal submucosa (SIS) is widely used to treat acute and chronic wounds. However, efficacy SIS accelerate wound healing still needs be improved meet clinical demands. To tackle this problem, platelet-rich plasma (PRP) due its potency promote proliferation, migration adhesion target cells. In study, we applied PRP explore their effects on by evaluating re-epithelialization, collagen production, angiogenesis inflammatory response. Methods A 1 × 1-cm full-thickness defect was established in mice. Sixty mice were divided into four treatment groups: + SIS, PRP, control. On days 3, 5, 7, 10 14 post-surgery, tissue specimens harvested. Haematoxylin eosin, Masson’s trichrome, immunohistochemical immunofluorescence double staining visualize epidermal thickness, vascular regeneration inflammation. Results Wound contraction groups significantly greater, compared with other groups, 3 5 post-surgery. histological analysis showed higher expression day which associated thicker layer 14. addition, that CD31-positive blood vessels endothelial growth factor higher, control group. Furthermore, number M1 M2 macrophages alone, 3. dramatically decreased groups. The ratio 3.97 2.93 times group 4.56 3.37 group, respectively. Conclusion Co-administration has better effect promoting angiogenesis, re-epithelialization managing than either agent alone.