miR-125b-5p delivered by adipose-derived stem cell exosomes alleviates hypertrophic scarring by suppressing Smad2

• DOI: 10.1093/burnst/tkad064
• Publication Date: 2024-05-19T10:41:27Z
• Authors: Chaolei Xu; Hao Zhang; Chen Yang; Ying Wang; Kejia Wang; Rui Wang; Wei Zhang; Chao Li; Chenyang Tian; Chao Han; Mengyang Li; Xu Liu; Yunwei Wang; Yan Li; Jian Zhang; Yu Li; Liang Luo; Yage Shang; Lixia Zhang; Yuxi Chen; Kuo Shen; Dahai Hu
• Keywords: 0301 basic medicine; 03 medical and health sciences; Research Article

Hypertrophic scarring is the most serious and unmet challenge following burn trauma injury often leads to pain, itching even loss of function. However, demand for ideal scar prevention treatment difficult satisfy. We aimed discover effects mechanisms adipose-derived stem cell (ADSC) exosomes in hypertrophic scarring. ADSC were isolated from culture supernatant ADSCs identified by nanoparticle tracking analysis, transmission electron microscopy western blotting. The effect on wound healing formation was detected model BALB/c mice. myofibroblasts tissue viability, proliferation migration myofibroblasts. In addition, collagen fibrosis-related molecules also detected. To further disclose mechanism fibrosis myofibroblasts, we expression Smad2 normal skin regulatory Smad2. Injection bleomycin performed male mice establish an vivo while administered observe their protective effect. observed via hematoxylin eosin Masson staining related testing. this study, found that could not only speed up improve quality but prevent formation. inhibited such as α-smooth muscle actin, I (COL1) COL3 transdifferentiation verified highly expressed both fibroblasts, downregulated fibroblasts. Further analysis revealed microRNA-125b-5p (miR-125b-5p) binds 3' untranslated region Smad2, thus inhibiting its expression. experiments alleviate bleomycin-induced downregulate scars suppression specific delivery miR-125b-5p.