Biologic instability of pancreatic cancer xenografts in the nude mouse

• DOI: 10.1093/carcin/21.6.1121
• Publication Date: 2002-07-26T22:20:01Z
• Authors: Bruno M. Schmied; Alexis B. Ulrich; Hosei Matsuzaki; Tarek H. El-Metwally; Xianzhong Ding; Mirabella E. Fern...; Thomas E. Adrian; William G. Chaney; Surinder K. Batra; Parviz M. Pour
• Keywords: 0301 basic medicine; Galectin 3; Transplantation, Heterologous; Radioimmunoassay; Mice, Nude; Antigens, Differentiation; Immunohistochemistry; 3. Good health; ErbB Receptors; Pancreatic Neoplasms; Mice; 03 medical and health sciences; Genes, ras; Transforming Growth Factor beta; Cricetinae; Karyotyping; Tumor Cells, Cultured; Animals; Humans; Female; Protein Binding

Tumor transplants into nude mice (NM) may reveal abnormal biological behavior compared with the original tumor. Despite this, human tumor xenografts in NM have been widely used to study biology of tumors and establish diagnostic therapeutic modalities. Clearly, precise differences a given cannot be assessed. We growth kinetics, differentiation pattern karyotype an anaplastic Syrian hamster pancreatic cancer cell line allogenic hamsters. As tumor, hamsters grew fast, were expressed markers related malignancy like galectin 3, TGF-alpha its receptor EGFR at high levels. However, well-differentiated adenocarcinomas, slower, had increased apoptotic rate expression such as blood group A antigen, DU-PAN-2, carbonic anhydrase II, TGF-beta(2) mucin. Karyotypically, acquired additional chromosomal damage. Our results demonstrate significant morphology grown host, call for caution extrapolating data obtained from primary cancer.