Calotropis procera Selectively Impaired the 4T1 Breast Cancer Cells Growth by Preferentially Blocking Akt/mTOR Signaling

• DOI: 10.1093/cdn/nzab036_020
• Publication Date: 2021-06-07T04:44:08Z
• Authors: Ana Carolina Silv...; Maria Angelica Mi...; Shirley Arbizu; Susanne Talcott; Ana Cláudia Carreira; Antonio Jose Cant...; Fernando Jose Cos...; Giuliana Noratto
• Keywords: Viability assay; Taxifolin

To investigate the mechanisms underlying anticancer activity of Calotropis procera crude phenolics extract (CphE). CphE were obtained from leaves homogenized with ethanol (1g:150 mL), followed by filtration and evaporation using a rotary evaporator. Quercetin was used as positive control since is one major flavonoids in C. procera. 4T1 cells treated (31–500 µg gallic acid equivalent (GAE)/mL), quercetin (Q) (0.6–3 µg/mL) or DMSO (control) to assess cell viability resazurin kit reactive oxygen species (ROS) Carboxy-H2DFFDA probe (Sigma-Aldrich, St Louis, MO). Protein mRNA expression investigated standard procedures migration wound healing assay. inhibited (within 31–125 GAE/mL) Q dose-dependent manner, IC50 = 49.6 GAE/mL 1,75 µg/mL, respectively. However, ROS levels decreased (down 0.7-fold control) while induced (up 1.5-fold control). These results suggest contrasting response breast cancer individual present CphE. The CphE-induced caspase PARP-dependent apoptosis suppression mediated CphE-mediated oxidative stress reduction consistent phospho-ERK1/2 downregulation 0.4-fold Conversely, apoptotic are induction ROS-phospho-ERK1/2 1.6-fold axis. Akt/mTOR/CREB pathway downregulated at similar extend Q, (suppressed 40% 20% respectively) protein phospho-Src (downregulated ∼ 0.2-fold phospho-CREB (0.7-fold 0.6-fold viability, reduced migration. effects result modulation proteins that play an important role epithelial-mesenchymal transition invasion. findings provide new insights into anti-cancer promising herb folk medicine for treatment. Coordenação de Aperfeiçoamento Pessoal Nível Superior (CAPES); Universidade São Paulo (USP).