Developmental Expression of Estrogen Receptor mRNA in the Rat Cerebral Cortex: A Nonisotopic in situ Hybridization Histochemistry Study

• DOI: 10.1093/cercor/2.1.1
• Publication Date: 2007-01-04T08:26:28Z
• Authors: Rajesh C. Miranda; C. Dominique Tora...
• Keywords: Cerebral Cortex; Male; 0301 basic medicine; Aging; Nucleic Acid Hybridization; Rats, Inbred Strains; Oligonucleotides, Antisense; Immunohistochemistry; Rats; Embryonic and Fetal Development; 03 medical and health sciences; Receptors, Estrogen; Pregnancy; Animals; Autoradiography; Female; RNA, Messenger; Oligonucleotide Probes; Phosphorus Radioisotopes

The distribution of estrogen receptor mRNA expression was studied in the developing rat cerebral cortex by situ hybridization histochemistry. We used a specific, nonisotopically (digoxigenin) labeled, synthetic oligo deoxyribonucleotide complementary to 48 base se quence region estrogen-binding domain uterine cDNA. During develop ment, observed all fore brain regions previously reported bind estrogen, as determined steroid autoradiography or nuclear ing assay. In cortex, extensively expressed ventricular zone, primitive plexiform layer, and immature cortical plate at least early embryonic day 16. first 3 postnatal weeks, ex pression increasingly restricted upper third neurons subplate (layer VIb/VIl) decreased low levels 28. spatial overlapped that for encoded protein. extensive throughout late prenatal points an important role differentiation maturation cortex.