Quantitative Applications of Fluorescence and Ultraviolet Scanning Densitometry for Compositional Analysis of Petroleum Products in Thin-Layer Chromatography

• DOI: 10.1093/chromsci/37.6.219
• Publication Date: 2013-04-24T04:54:33Z
• Authors: V. L. Cebolla; L. Membrado; M. P. Domingo; P. Henrion; R. Garriga; P. Gonzalez; F. P. Cossio; A. Arrieta; J. Vela
• Keywords: Petrochemical; Densitometry

Direct application of the fluorescence response enhancement (derived from interaction berberine cation with saturated hydrocarbons) to petrochemical analysis is achieved. Hydrocarbon types are successfully determined in middle (gasoil) and heavy (lubricants, vis-breaking fuel, oil) petroleum distillates adequate precision sensitivity. The novelty this method resides fact that a single scanning only needed for determination saturates aromatics same chromatogram. In spite their chemical inertness, hydrocarbons can be quantitatively using thin-layer chromatographic densitometry. fluorescent depends on alkane structure. A reliable choice variables influence response, precision, sensitivity: sample load, volume, beam size, impregnation conditions (berberine concentration time). Sensitivity tailored certain extent through control last parameter. quantitative results agree those provided other well-established techniques industry.