Chromatographic Estimation of p-Coumaric Acid and Triacontanol in an Ayurvedic Root Drug Patala (Stereospermum suaveolens Roxb.)

• DOI: 10.1093/chromsci/47.10.936
• Publication Date: 2013-04-24T09:42:12Z
• Authors: N. Srivastava; S. Khatoon; A. K. S. Rawat; V. Rai; S. Mehrotra
• Keywords: 0301 basic medicine; 03 medical and health sciences; Coumaric Acids; Plant Extracts; Bignoniaceae; Fatty Alcohols; Propionates; Plant Roots; Chromatography, High Pressure Liquid; Medicine, Ayurvedic

A high-performance thin layer chromatographic method has been established for the identification and standardization of Stereospermum suaveolens extracts using two active biomarkers viz. triacontanol and p-coumaric acid in root samples collected from different geographical locations of India. These marker components have different therapeutic activities: p-coumaric acid is a good antioxidant and also has anti-diabetic properties; triacontanol has anti-inflammatory and anti-ulcer properties. Studies on root as well as stem samples showed characteristic bands of triacontanol and p-coumaric acid at R(f) 0.51 and 0.42, respectively. The amount of triacontanol varied from 0.035-8.472 mg/g in the root samples while the amount of p-coumaric acid was in the range 0.081-0.203 mg/g. The limit of detection for triacontanol was 100 ng/spot whereas that for p-coumaric acid was 10 ng/spot.