Larval excretion/secretion of dipters of Lucilia cuprina species induces death in promastigote and amastigote forms of Leishmania amazonensis

• DOI: 10.1093/femspd/ftab040
• Publication Date: 2021-08-02T19:13:29Z
• Authors: Ana Carolina Jaco...; Bruna Taciane da ...; Amanda Cristina M...; Taylon Felipe Silva; Virgínia Márcia C...; Manoela Daiele Go...; Fernanda Tomiotto...; Mariana Barbosa D...; Andrea Diaz-Roa; Pedro Ismael da S...; Idessania Nazaret...; Ivete Conchon-Costa; Danielle Lazarin ...; Milena Menegazzo ...; Wander Rogério Pa...
• Keywords: Amastigote; Lucilia cuprina

Leishmaniasis is a neglected tropical disease that affects millions of people around the world. Larval excretion/secretion (ES) larvae flies Calliphoridae family has microbicidal activity against Gram-positive and Gram-negative bacteria, in addition to some species Leishmania. Our study aimed at assessing vitro efficacy Lucilia cuprina larval ES promastigote amastigote forms Leishmania amazonensis, elucidating possible mechanisms routes death involved. was able inhibit viability L. amazonensis all concentrations, induce morphological ultrastructural changes parasite, retraction cell body, roughness cytoplasmic membrane, leakage intracellular content, ROS production increase, induction membrane depolarization mitochondrial swelling, formation lipid droplets phosphatidylserine exposure, thus indicating possibility apoptosis-like death. To verify on forms, we performed phagocytic assay, measurement total NO. Treatment using reduced percentage infection number amastigotes per macrophage lineage J774A.1 increasing TNF-α, pro-inflammatory immunomodulation oxidative damage. Therefore, treatment effective inducing promastigotes even low concentrations.