Characterization of bacteriophage vB_AbaS_SA1 and its synergistic effects with antibiotics against clinical multidrug-resistant Acinetobacter baumannii isolates

• DOI: 10.1093/femspd/ftae028
• Publication Date: 2024-10-22T09:57:07Z
• Authors: Sanaz Rastegar; Salehe Sabouri; Omid Tadjrobehkar; Ali Samareh; Hira Niaz; Nafise Sanjari; Hossein Hosseini-...; Mikael Skurnik
• Keywords: Acinetobacter baumannii; Phage therapy

Abstract Acinetobacter baumannii is a major cause of nosocomial infections globally. The increasing prevalence multidrug-resistant (MDR) A. has become an important public health concern. To combat drug resistance, alternative methods such as phage therapy have been suggested. In total, 30 MDR strains were isolated from clinical specimens, and their antibiotic susceptibilities determined. vB_AbaS_SA1, hospital sewage, was characterized. addition to its plaque size, particle morphology, host range, genome sequence determined annotated. Finally, the antibacterial effects alone, antibiotics phage/antibiotic combinations assessed against strains. Phage vB_AbaS_SA1 had siphovirus showed latent period 20 min, 250 PFU/cell (plaque forming unit/cell) burst size. When combined with antibiotics, (SA1) significant phage-antibiotic synergy effect reduced overall effective concentration in time-kill assessments. SA1 linear double-stranded DNA 50 108 bp size guanine-cytosine (GC) content 39.15%. Despite potent SA1, it necessary perform additional research completely elucidate mechanisms action potential constraints associated utilizing this bacteriophage.