Sequential Colonization of Periodontal Pathogens in Induction of Periodontal Disease and Atherosclerosis in LDLR null Mice

• DOI: 10.1093/femspd/ftx003
• Publication Date: 2017-01-20T01:15:12Z
• Authors: Sasanka S. Chukka...; Meena Easwaran; Mercedes F. River...; Irina M. Velsko; Sriram Ambadapadi; Jiayin Dai; Hannu Larjava; Alexandra R. Lucas; Lakshmyya Kesavalu
• Keywords: Tannerella forsythia; Treponema denticola; Fusobacterium nucleatum; Dental plaque; Periodontal pathogen

Periodontal disease (PD) and atherosclerotic vascular (ASVD) are both chronic inflammatory diseases with a polymicrobial etiology have been epidemiologically associated. The purpose is to examine whether periodontal bacteria that infect the periodontium can also tissues enhance pre-existing early aortic lesions in LDLRnull mice. Mice were orally infected intermediate bacterial colonizer Fusobacterium nucleatum for first 12 weeks followed by late colonizers (Porphyromonas gingivalis, Treponema denticola Tannerella forsythia) remaining mimicking human oral microbiota ecological colonization. Genomic DNA from all four was detected gingival plaque PCR, consistently demonstrating infection of mouse surfaces. Infected mice had significant levels IgG IgM antibodies, alveolar bone resorption, showed apical migration junctional epithelium revealing induction PD. These results support ability cause PD Detection genomic systemic organs indicates hematogenous dissemination pockets. Bacterial did not alter serum lipid fractions or amyloid A induce plaque. This study examining causal role ASVD