Flow-cytometric analysis of human monocyte subsets targeted byMycobacterium bovisBCG before granuloma formation

• DOI: 10.1093/femspd/fty080
• Publication Date: 2018-11-15T16:21:32Z
• Authors: Melaine Delcroix; Kartoosh Heydari; Ren Dodge; Lee W Riley
• Keywords: Monocyte; CD16; Infectivity

Infection with Mycobacterium tuberculosis (Mtb) is characterized by an inflammatory response resulting in the formation of granulomas. These tight aggregates immune cells play important role bacterial containment and eventual outcome infection. Monocytes are a major component early to Mtb contribute cellular matrix newly forming granuloma. Therefore, defining which monocyte subset target mycobacterial infection critical. Here, we describe flow-cytometry-based assay analyze infectivity vitro subsets bovis BCG before granuloma formation. We identified CD14+CD16− monocytes as main peripheral blood mononuclear from six healthy donors. CD14+CD16+ displayed lowest rates remained uninfected some found that longer time resulted increase percentage infected number granulomas produced. did not observe changes cell death or expansion upon Future experiments our method could help define subsets. Our study provides platform investigate how different may alter outcomes