Interactions Between the Inositol 1,4,5-Trisphosphate and Cyclic AMP Signaling Pathways Regulate Larval Molting in Drosophila

• DOI: 10.1093/genetics/158.1.309
• Publication Date: 2021-04-28T05:36:16Z
• Authors: K Venkatesh; G Siddhartha; Rohit Joshi; Sonal Patel; Gaiti Hasan
• Keywords: 0303 health sciences; Receptors, Cytoplasmic and Nuclear; Inositol 1,4,5-Trisphosphate; Cyclic AMP-Dependent Protein Kinases; Animals, Genetically Modified; 03 medical and health sciences; Larva; Mutation; Cyclic AMP; Animals; Inositol 1,4,5-Trisphosphate Receptors; Drosophila; Calcium Channels; Alleles; Adenylyl Cyclases; Signal Transduction

Larval molting in Drosophila, as other insects, is initiated by the coordinated release of steroid hormone ecdysone, response to neural signals, at precise stages during development. In this study we have analyzed, using genetic and molecular methods, roles played two major signaling pathways regulation larval Drosophila. Previous studies shown that mutants for inositol 1,4,5-trisphosphate receptor gene (itpr) are lethals. addition they exhibit delays can be rescued exogenous feeding 20-hydroxyecdysone. Here show adenylate cyclase (rut) synergize, molting, with itpr mutant alleles, indicating both cAMP InsP(3) function process. The act parallel affect judged phenotypes obtained through expression dominant negative active forms protein kinase A (PKA) tissues normally express receptor. Furthermore, our predict existence feedback inhibition on increased levels