Sex-related differences in the developmental rate of in-vitro matured/in-vitro fertilized ovine embryos

• DOI: 10.1093/humrep/11.3.621
• Publication Date: 2012-04-06T02:55:06Z
• Authors: M.L. Bernardi; C. Delouis
• Keywords: Male; Sex Characteristics; Sex Determination Analysis; Sheep; [SDV]Life Sciences [q-bio]; Fertilization in Vitro; Polymerase Chain Reaction; [SDV] Life Sciences [q-bio]; Embryonic and Fetal Development; 03 medical and health sciences; 0302 clinical medicine; 5. Gender equality; Y Chromosome; Animals; Female; Sex Ratio

Sex determination of in-vitro matured/in-vitro fertilized ovine embryos cultured in synthetic oviduct fluid (SOF) medium was performed by the polymerase chain reaction amplification specific Y DNA sequences so as to test influence sex on developmental growth during preimplantation period. At 144 h post-insemination, with a blastocoel were classified fast-developing group, whereas those showing only after this length time slow-developing group. separately and some according size their blastocoel. end culture (207 post-insemination), all both stage morphological quality. The male: female ratio significantly higher than expected 50%. More males observed at most advanced 207 post-insemination. proportion did not differ between good- poor-quality groups, although skewed better quality developed stage. In conclusion, predominantly male derived mainly from raising possibility deviation transfer embryos.