Inducible expression of a CC chemokine liver- and activation-regulated chemokine (LARC)/macrophage inflammatory protein (MIP)-3α/CCL20 by epidermal keratinocytes and its role in atopic dermatitis

• DOI: 10.1093/intimm/13.1.95
• Publication Date: 2002-07-26T22:50:44Z
• Authors: Takashi Nakayama; Ryuichi Fujisawa; Hidekazu Yamada; Tatsuya Horikawa; Hiroshi Kawasaki; Kunio Hieshima; Dai Izawa; Satoru Fujiie; Tadashi Tezuka; Osamu Yoshie
• Keywords: CCL20; Proinflammatory cytokine

Liverand activation-regulated chemokine (LARC)/macrophage inflammatory protein (MIP)-3α/CCL20 is a CC which constitutively expressed by follicle-associated epithelial cells in the mucosa, and attracts expressing CCR6 such as immature dendritic α4β7high intestine-seeking memory T cells. Here, we examine LARC/CCL20 expression skin. mRNA were induced primary human keratinocytes upon stimulation with proinflammatory cytokines IL-1α tumor necrosis factor (TNF)-α. In mice, intradermal injection of TNF-α rapidly local accumulation transcripts for its receptor lag several hours latter. humans, immunostaining was weak if any normal skin tissues but strongly augmented lesional atopic dermatitis. Furthermore, massive infiltration markers CD1a, CD3 or HLA-DR present lesions. Many infiltrating also found to be CCR6+ newly generated monoclonal anti-CCR6. However, Langerhans residing within epidermis hardly stained anti-CCR6 tissues. plasma levels elevated patients Collectively, our results suggest that epidermal produce TNF-α, attract CCR6-expressing memory/effector into dermis inflamed may not, however, play major role homeostatic migration