Insecticidal Activity of Avidin Combined with Genetically Engineered and Traditional Host Plant Resistance Against Colorado Potato Beetle (Coleoptera: Chrysomelidae) Larvae

• DOI: 10.1093/jee/99.2.527
• Publication Date: 2015-01-13T19:31:32Z
• Authors: Susannah G. Cooper; David S. Douches; Edward J. Grafius
• Keywords: Colorado potato beetle; Leptinotarsa; Avidin

Colorado potato beetle, Leptinotarsa decemlineata (Say), is a destructive pest of potato, Solanum tuberosum (L.), in North America. It renowned for adapting to insecticides. With the arsenal effective insecticides decreasing, it important consider alternative forms control. Biotin an essential coenzyme insect growth and development. Avidin protein found chicken egg that sequesters biotin has shown insecticidal properties against range insect. We assessed effectiveness avidin beetle neonates no-choice detached leaf bioassay at 0, 17, 34, 51, 102, 204 μg avidin/ml over 12 d. The LC50 was 136 (108–188 95% CL). combined effects (136 avidin/ml) with Bt-Cry3A or leptines were evaluated third instars 6 d, respectively. Three lines used: susceptible line, line engineered express Cry3A from Bacillus thuringiensis, expressing natural resistance factor leptines. addition concentration significantly reduced consumption by neonates, but did not affect feeding on leptine line. Survival compared after 3 d survival Bt-Cry3A, suggesting may increase susceptibility Bt-Cry3A.