Draftde novoGenome Assembly of the Elusive Jaguarundi,Puma yagouaroundi

• DOI: 10.1093/jhered/esab036
• Publication Date: 2021-06-17T19:11:44Z
• Authors: Gaik Tamazian; Pavel Dobrynin; Anna Zhuk; Daria V Zhernakova; Polina L Perelman; Natalia A Serdyukova; Alexander S Graph...; Aleksey Komissarov; Sergei Kliver; Nikolay Cherkasov; Alan F Scott; David W Mohr; Klaus-Peter Koepfli; Stephen J O’Brien; Ksenia Krasheninn...
• Keywords: Puma

Abstract The Puma lineage within the family Felidae consists of 3 species that last shared a common ancestor around 4.9 million years ago. Whole-genome sequences 2 from were previously reported: cheetah (Acinonyx jubatus) and mountain lion (Puma concolor). present report describes whole-genome assembly remaining species, jaguarundi yagouaroundi). We sequenced genome male with 10X Genomics linked reads assembled sequence. contains series scaffolds reach length chromosome arms is similar in scaffold contiguity to assemblies puma, contig N50 = 100.2 kbp 49.27 Mbp. assessed sequence using BUSCO, aligned individual another published female genome, annotated protein-coding genes, repeats, genomic variants their effects respect analyzed differences jaguarundis reference mitochondrial genome. its annotation compared quality, variants, features reported puma cheetah. Computational analyzes used study implemented transparent reproducible way allow further reuse modification.