The orphan G protein–coupled receptor 141 expressed in myeloid cells functions as an inflammation suppressor

• DOI: 10.1093/jleuko/qiae009
• Publication Date: 2024-01-16T12:07:05Z
• Authors: Atsuya Sawabe; Shogo Okazaki; Akira Nakamura; Ryo Goitsuka; Tomonori Kaifu
• Keywords: Orphan receptor

Abstract G protein–coupled receptors (GPCRs) regulate many cellular processes in response to various stimuli, including light, hormones, neurotransmitters, and odorants, some of which play critical roles innate adaptive immune responses. However, the physiological functions GPCRs involvement them autoimmune diseases central nervous system remain unclear. Here, we demonstrate that GPR141, an orphan GPCR belonging class A receptor family, suppresses High GPR141 messenger RNA levels were expressed myeloid-lineage cells, neutrophils (CD11b + Gr1+), monocytes Gr1−Ly6C+ CD11b Gr1−Ly6C−), macrophages (F4/80+), dendritic cells (CD11c+). Gpr141 −/− mice, independently generated, displayed almost no abnormalities myeloid cell differentiation compartmentalization spleen bone marrow under steady-state conditions. deficiency exacerbated disease conditions experimental encephalomyelitis, model for multiple sclerosis, with increased inflammation spinal cord. mice showed Gr1+ neutrophils, Gr1− monocytes, CD11c+ CD4+ T infiltration into encephalomyelitis–induced cord compared littermate control mice. Lymphocytes enriched from immunized myelin oligodendrocyte glycoprotein 35–55 produced high amounts interferon-γ, interleukin-17A, interleukin-6 those wild-type Moreover, (DCs) purified cytokine production 35–55–specific cells. These findings suggest as a negative regulator responses by controlling targeting may be possible therapeutic intervention modulating chronic inflammatory diseases.