Neutrophil extracellular traps capture the human pathogen, Candida albicans, in blood and delay hyphal transformation

• DOI: 10.1093/jleuko/qiaf002
• Publication Date: 2025-01-03T12:16:12Z
• Authors: Miyuki Sakuma; Adam Viens; Alex Hopke; Daniel J Floyd; Musie Ghebremichael; Michael K Mansour; Daniel Irimia
• Keywords: Neutrophil Extracellular Traps

Abstract In tissues, neutrophils neutralize Candida albicans through phagocytosis and delay C. hyphae growth by deploying neutrophil extracellular traps (NETs). However, in the bloodstream, dynamic interactions between NETs are far less understood. Here, we employ a microfluidic assay measure significant increase intact blood within 3 hours after adding yeast or hyphae. We show that can be captured efficiently on NETs, thereby delaying transition to hyphal growth. higher amounts of samples from invasive candidiasis patients compared healthy participants, both with without stimulation. These findings suggest bloodstream stimulate NET release, potentially aiding their removal blood.