Effects of leguminous green manure–crop rotation on soil enzyme activity and stoichiometry

• DOI: 10.1093/jpe/rtae065
• Publication Date: 2024-07-26T12:54:32Z
• Authors: Meixia Liu; Zonghao Hu; Yi Fan; Bin Hua; Wei Yang; Shuang Pang; Rong Mao; Yang Zhang; Keyu Bai; Carlo Fadda; Paola De Santis; Nadia Bergamini; Aziza Usmankulova; Buriyev Salimjan ...; Ximei Zhang
• Keywords: Topsoil; Soil carbon; Crop Rotation

Abstract As a crucial strategy for sustainable agricultural production, green manure–crop rotation can regulate soil nutrient cycling and decrease the reliance on nitrogen fertilizers. However, we still lack comprehensive understanding of changes in eco-enzyme activities, microbial metabolism limitations caused by leguminous rotation. Here, conducted field experiments across China to analyze extracellular enzyme specifically β-glucosidase (BG), N-acetyl-β-d-glucosaminidase (NAG), leucine aminopeptidase (LAP) acid phosphatase (AP). The study revealed that long-term increased carbon accumulation farmland, with significant average increase 20.1% 36.4% BG, AP activities topsoil, while showing ln(NAG + LAP):ln(AP) ratios. ratios ln(BG):ln(NAG LAP) various regions were typically below 1:1, indicating activity is more constrained phosphorus nutrients rather than carbon. Precipitation, temperature, total (TC) (TN) identified as key environmental factors stoichiometric Our highlights alleviates limitation enhancing limitation, closely related TC TN soil.