Ginsenoside Rg3 inhibits renal cell carcinoma cell migration, invasion, colony formation, and tube formation and enhances apoptosis through promoting the DNA demethylation and histone acetylation

• DOI: 10.1093/jpp/rgac072
• Publication Date: 2022-10-20T18:01:25Z
• Authors: Zhiqiang Ma; Yan Zuo; Wei Wang
• Keywords: Histone deacetylase inhibitor; Histone H4

This study explored the effect and mechanism of Rg3 on renal cell carcinoma (RCC) progression.RCC cells were treated with different concentrations Rg3, 5-Aza-dc (a methyltransferase inhibitor) or TSA deacetylase inhibitor). Rg3-induced cytotoxicity, migration, invasion, colony formation, tube formation apoptosis RCC evaluated by CCK-8, wound healing, Transwell, flow cytometry assays, respectively. Methylation expressions p53, p21 p16, methylation-related genes histone deacetylases acetylation-related (H3 (acetyl K14), H3 K9), H4 K12), K5) K16)) analysed qRT-PCR western blot.Rg3 dose-dependently decreased viability, inhibited enhanced cells. demethylation levels p16 as well genes, but repressed deacetylases. had same did above-mentioned cellular changes.Rg3 restrains yet enhances through promoting acetylation.