P sequences ofDrosophilla Subobscuralack exon 3 and may encode a 66 kd repressor-like protein

• DOI: 10.1093/nar/19.24.6713
• Publication Date: 2007-01-05T02:27:37Z
• Authors: Nuria Paricio; Manuel Pèrez-Alonso; maria Josè Martin...; Rosa de Frutos
• Keywords: Leucine Zippers; Base Sequence; Molecular Sequence Data; Restriction Mapping; Nucleic Acid Hybridization; Transposases; Exons; Nucleotidyltransferases; Repressor Proteins; Sequence Homology, Nucleic Acid; DNA Transposable Elements; Animals; Drosophila; Amino Acid Sequence; Cloning, Molecular; Repetitive Sequences, Nucleic Acid

Several P homologous sequences have been cloned and sequenced from Drosophila subobscura. These sequences are located at the 85DE region of the O chromosome and at least three of them are organized in tandem. We have identified four copies which exhibit strong similarity between them. All of the isolated elements are truncated at the 5' and 3' ends. They have lost the inverted terminal repeats and exon 3, but maintain exons 0, 1 and 2. They are transcribed producing a polyadenylated RNA. The structure of these transcripts suggests that they are able to encode a 66 kd repressor-like protein, but not a functional transposase. We ask about the biological role of a potential repressor protein in this species.