Systematic comparison of gene expression through analysis of cDNA fragments within or near to the protein-coding region

• DOI: 10.1093/nar/27.3.912
• Publication Date: 2002-07-26T18:40:19Z
• Authors: Y. Ke; C. Jing; P. H. Smith; C. S. Foster; P. S. Rudland
• Keywords: Coding region

Life is controlled by the timely and ordered expression of genes. Identification important genes involved in specific physiological pathological conditions requires efficient methods to analyse differential gene expression. We describe a novel strategy, namely complete comparison (CCGE), for systematic assessment differentially expressed Using CCGE method, double-stranded cDNA digested with two restriction enzymes that cut different frequencies, representative fragments are generated within or near proteincoding region. After being flanked types adapters, amplified nested suppression PCR, selected fragments, representing entire population, can be divided into 256 subsets; compared manner.