ATM controls meiotic DNA double-strand break formation and recombination and affects synaptonemal complex organization in plants

• DOI: 10.1093/plcell/koab045
• Publication Date: 2021-02-03T16:46:10Z
• Authors: Marie-Therese Kur...; Michael Peter Jan...; Luis F Paulin; Ignacio Prusén Mota; Konstantin Tomanov; Ondrej Krsicka; Arndt von Haeseler; Veit Schubert; Peter Schlögelhofer
• Keywords: Synaptonemal complex; Synapsis; Chromosomal crossover

Meiosis is a specialized cell division that gives rise to genetically distinct gametic cells. relies on the tightly controlled formation of DNA double-strand breaks (DSBs) and their repair via homologous recombination for correct chromosome segregation. Like all forms damage, meiotic DSBs are potentially harmful activates an elaborate response inhibit excessive break ensure successful repair. Previous studies established protein kinase ATM as DSB sensor regulator in several organisms. Here we show Arabidopsis acts at multiple steps during processing, well crossover (CO) synaptonemal complex (SC) organization, vital completion meiosis. We developed single-molecule approach quantify determined essential limit number DSBs. Local genome-wide screens showed restricts interference-insensitive COs, while super-resolution STED nanoscopy chromosomes revealed affects chromatin loop size SC length width. Our study extends our understanding how functions plant meiosis establishes it integral factor program.