A proxitome-RNA-capture approach reveals that processing bodies repress coregulated hub genes

• DOI: 10.1093/plcell/koad288
• Publication Date: 2023-11-16T18:00:43Z
• Authors: Chen Liu; Andriani Mentzelo...; Ioannis H Hatzian...; Epameinondas Tzag...; Vasileios Skaltso...; Xuemin Ma; Vassiliki A Micha...; Francisco J Romer...; Ana B Romero-Losada; Panagiotis F Sarris; Peter Marhavy; Bettina Bölter; Alexandros Kanter...; Emilio Gutierrez-...; Panagiotis N Moschou
• Keywords: Large-Scale Biology; Arabidopsis; RNA; RNA-Binding Proteins; Processing Bodies; Heat-Shock Response

Cellular condensates are usually ribonucleoprotein assemblies with liquid- or solid-like properties. Because these subcellular structures lack a delineating membrane, determining their compositions is difficult. Here we describe proximity-biotinylation approach for capturing the RNAs of known as processing bodies (PBs) in Arabidopsis (Arabidopsis thaliana). By combining this RNA detection, silico, and high-resolution imaging approaches, studied PBs under normal conditions heat stress. showed much more dynamic composition than total transcriptome. involved cell wall development regeneration, plant hormonal signaling, secondary metabolism/defense, metabolism were enriched PBs. RNA-binding proteins liquidity modulated recruitment, while frequently recruited together encoded proteins. In PBs, follow distinct fates: small liquid-like get degraded larger ones, they stored. PB properties can be regulated by actin-polymerizing SCAR (suppressor cyclic AMP)-WAVE (WASP family verprolin homologous) complex. SCAR/WAVE modulates shuttling between translational machinery, thereby adjusting ethylene signaling. summary, provide an to identify that allowed us reveal mechanism regulating fate.