Nonspecific phospholipases C3 and C4 interact with PIN-FORMED2 to regulate growth and tropic responses in Arabidopsis

• DOI: 10.1093/plcell/koae071
• Publication Date: 2024-03-05T20:59:51Z
• Authors: Teng Li; Shujuan Zhang; Shuaibing Yao; Xuebing Li; Qianru Jia; Jingya Yuan; Wenhua Zhang; Xuemin Wang; Qun Zhang
• Keywords: 0303 health sciences; Indoleacetic Acids; Arabidopsis Proteins; Arabidopsis; Phosphatidic Acids; Plants, Genetically Modified; Plant Roots; Endocytosis; Gravitropism; 03 medical and health sciences; Gene Expression Regulation, Plant; Type C Phospholipases; Signal Transduction

The dynamic changes in membrane phospholipids affect biophysical properties and cell signaling, thereby influencing numerous biological processes. Nonspecific phospholipase C (NPC) enzymes hydrolyze common to release diacylglycerol (DAG), which is converted phosphatidic acid (PA) other lipids. In this study, 2 Arabidopsis (Arabidopsis thaliana) tandemly arrayed genes, NPC3 NPC4, were identified as critical factors modulating auxin-controlled plant growth tropic responses. Moreover, NPC4 shown interact with the auxin efflux transporter PIN-FORMED2 (PIN2). loss of enhanced endocytosis vacuolar degradation PIN2, disrupted gradients slowed gravitropic halotropic Furthermore, auxin-triggered activation required for asymmetric PA distribution that controls PIN2 trafficking dynamics auxin-dependent Collectively, our study reveals an NPC-derived signaling pathway fluxes essential fine-tuning balance between root environmental